Genomic Analysis

Cross references:  Genetics in General    Genomics in General  

A practical approach for quantitating specific mRNAs by solution hybridization
(Goog) 
Only abstract available online.  I got the PDF through the library. 
    "
The preparation and use of a specific cDNA probe for quantitating mRNA by solution hybridization is described. Cloned DNA sequences are nick translated, denatured, hybridized to single-stranded M13 clones containing message strand (mDNA) sequences, and separated chromatographically on Bio-Gel A50 under first native and then denaturing conditions to yield a single-stranded cDNA probe. The details of a solution hybridization assay in which the single-stranded cDNA is used to quantitate mRNA in total nucleic acid samples are described. As little as 0.5 pg of mRNA can easily be detected within a day of sample isolation. Thus, the assay is both rapid and sensitive and can be used to measure RNAs complementary to any cloned DNA sequence. It is ideally suited to situations when accurate quantitation of multiple samples is anticipated.

My comment
Since this article was published in 1983, the technology has been available for a long time.  The author gives "mRNA quantitation
" as key words, and I may come back to look at this more closely.  
 


Ancestral gene resurrection - Wikipedia .  
https://en.wikipedia.org/wiki/Ancestral_gene_resurrection  
     "Ancestral gene resurrection is a technique that belongs to the study of molecular evolution. The method consists in the synthesis of an ancestral gene and expression of the corresponding ancestral protein.[1] The idea of protein resurrection was suggested in 1963 by Pauling and Zuckerkandl.[2] Some early efforts were made in the nineties, led by the laboratory of Steven A. Benner, a biochemist at the Swiss Federal Institute of Technology and later the University of Florida.[3] Thanks to the improvement of algorithms and of better sequencing and synthesis techniques, the method was developed further in the early 2000s to allow the resurrection of much more ancient genes.[4] Over the next decade, ancestral protein resurrection was developed as a strategy to reveal the mechanisms and dynamics of protein evolution, led mainly by Joseph Thornton, an evolutionary biologist at the University of Oregon.[5]

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